Antibacterial and Cytotoxic Activities of Ascidians Polyclinum madrasensis Sebestian, 1952 and Phallusia nigra Savigny, 1816 from Tuticorin Coast of India

Ascidians are involved with rich source of bioactive agents which could be used for novel antimicrobial drugs. Ascidians are belongs to phylum chordate and class Ascidiacea. In present study two ascidians P. madrasensis and P. nigra were collected from Tuticorin, Southeast coast of India. The antibacterial compounds were screened to evaluate antibacterial activity in and different solvent like crude, methanol, dichloromethane, 2-ethoxy ethanol, acetone, chloroform, n-butanol, ethyl acetate and diethyl ether. In antibacterial activity S. aureus shows most sensitive against Phallusia nigra n-butanol extracts (17 ± 0.124 mm). Except for the 2ethoxy ethanol, n-butanol, ethyl acetate and diethyl ether extracts of P. madrasensis against S. aureus, K. oxytoca, V. cholerae, E. coli and P. mirabilis. The cytotoxic activity of P. madrasensis and P. nigra extracts have been tested at various concentrations and showed the results of highest cytotoxicity assay conducted, indicating the presence of cytotoxic compounds in these ascidians.